Holographic fast gradient light interference microscopy (HF-GLIM)

Sakakura, Masayoshi

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https://hdl.handle.net/2142/107235 Copy

Description

Title

Holographic fast gradient light interference microscopy (HF-GLIM)

Author(s)

Sakakura, Masayoshi

Contributor(s)

Popescu, Gabriel

Issue Date

2020-05

Keyword(s)

• Holography
• Quantitative phase imaging
• Sagnac interferometer
• Gradient light interference microscopy
• Differential interference contrast microscopy

Abstract

Quantitative phase imaging has been an emerging technique in the field of biomedical imaging. As one type, gradient light interference microscopy (GLIM) is used to retrieve a quantitative gradient mapping of the phase delay that is produced by a biological sample. The method involves collecting four phase-shifted frames for a single field of view, which ultimately combines arithmetically into phase gradient information. The phase-shifting is done by a space light modulator (SLM) which intakes an appropriate voltage that converts into a corresponding phase retardation in the image field. Despite GLIM’s ability to generate a quantitative phase gradient of a sample, such module depends heavily on the operational speed of the SLM. The holographic fast gradient light interference microscopy (HF-GLIM) aims to retrieve a field of view with only a single image from the conventional differential interference contrast microscope (DIC), and is sent to a tilted Sagnac interferometer to create a holographic output at the camera. Through digital processing of the Hologram using Fourier filtering, the phase gradient that is given by the sample can be retrieved only through a single recording instance from the HF-GLIM. The publication aims to derive the functionality of HF-GLIM utilizing a mathematical derivation, geometrical analysis, and the simulation results.

Type of Resource

text

Language

en

Permalink

http://hdl.handle.net/2142/107235

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