Protein interactions in vitro and in the cell

Guin, Drishti

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https://hdl.handle.net/2142/106142 Copy

Description

Title

Protein interactions in vitro and in the cell

Author(s)

Guin, Drishti

Issue Date

2019-08-23

Director of Research (if dissertation) or Advisor (if thesis)

Gruebele, Martin

Doctoral Committee Chair(s)

Gruebele, Martin

Committee Member(s)

• Leckband, Deborah
• Freeman, Brian
• Shukla, Diwakar

Department of Study

Chemistry

Discipline

Chemistry

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

Ph.D.

Degree Level

Dissertation

Keyword(s)

Protein binding, in cell, in vitro, dodine, denaturant, protein interactions

Abstract

The cell is a complex crowded environment containing up to 300 mg/mL bio-macromolecules. In this environment proteins are far from isolated systems and interact with many other biological molecules they encounter. In recent years, better instrumentation and greater computing power have enabled imaging of elusive biomolecule dynamics in cells, driving many advances in understanding the chemical organization of biological systems. Interactions in the cell affect both biomolecular stability and function. The same protein or nucleic acid can behave differently depending on time in the cell cycle, location in a specific compartment, or stresses acting on the cell. My thesis focuses on these interactions both in vitro and in the cell and the differences between a model in vitro environment and the cell. The broader goal of my research is to emphasize the importance of the cellular environment in designing future biological studies.

Graduation Semester

2019-12

Type of Resource

text

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http://hdl.handle.net/2142/106142

Copyright and License Information

Copyright 2019 Drishti Guin

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