3D momentum imaging of laser desorption ionization of 2,5-dihydroxybenzoic acid (DHB)

Stewart, Gabriel A.

Permalink

https://hdl.handle.net/2142/104392 Copy

Description

Title

3D momentum imaging of laser desorption ionization of 2,5-dihydroxybenzoic acid (DHB)

Author(s)

Stewart, Gabriel A.

Contributor(s)

• Li, Wen
• Basnayake, Gihan
• Debrah, Duke A.

Issue Date

2019-06-20

Keyword(s)

Spectroscopy as an analytical tool

Abstract

Matrix-assisted laser desorption ionization (MALDI) is a widely used mass spectrometric technique for the mass analysis of biomolecular compounds. For over three decades since its development a considerable effort has been devoted to increasing the mass resolution and efficiency of MALDI. However, due to a lack of a detailed description of the fundamental processes, underlying the initial ionization, leaves optimization of the method at a trial-and-error endeavor. Generally, MALDI exploits a laser pulse to commence an ionization event where ions, neutrals, and electrons are ejected from the substrate surface into the gas phase (plume). Here we used 3D momentum imaging of laser desorption ionization to investigate the ionization dynamics of dihydroxybenzoic acid (DHB) with different laser pulse durations. Varying the pulse duration between femtoseconds and picoseconds present significantly different dynamics that are reflective of the velocity distributions. These findings suggest that MALDI is not a single molecule process, rather a collective intermolecular phenomenon.

Publisher

International Symposium on Molecular Spectroscopy

Type of Resource

text

Language

eng

Permalink

http://hdl.handle.net/2142/104392

DOI

https://doi.org/10.15278/isms.2019.RL09

Copyright and License Information

Copyright 2019 Gabriel A. Stewart

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