Director of Research (if dissertation) or Advisor (if thesis)
Gao, Liang
Department of Study
Electrical & Computer Eng
Discipline
Electrical & Computer Engr
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
M.S.
Degree Level
Thesis
Keyword(s)
FLIM
CUP
Abstract
Fluorescence lifetime imaging microscopy (FLIM) has been extensively applied in wide biomedical applications from single cell studies to medical diagnosis. While the state-of-the-art FLIM techniques are highly sensitive to biological dynamics such as fluorescence resonance emission transfer (FRET), they depend on repetitive measurements and the slow frame rate limits the study of ultrafast biological dynamics. This thesis reports the world’s fastest high-resolution FLIM at 100 frames per second (fps). This system, referred to as compressed FLIM, can capture a widefield lifetime image of 500 450 pixels within a single camera snapshot and operate real-time two-dimensional (2D) FLIM at 100 fps. By combining compressed ultrafast photography (CUP) with FLIM and adopting dual-camera detection, compressed FLIM is demonstrated for both fluorescent biological samples lifetime imaging as well as 75 fps image acquisition of fluorescent beads diffusion dynamics. Compressed FLIM holds great promise for quantitative live cell imaging applications. Compressed FLIM also has great potential in high-speed imaging of fluorescence lifetime in transient biological events such as FRET and may further extend quantitative live cell imaging such as monitoring neural spiking.
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