University of Illinois Urbana-Champaign

Mitochondrial genome in Hypsizygus marmoreus and its evolution in Dikarya

Wang, Gang; Lin, Jingxian; Shi, Yang; Chang, Xiaoguang; Wang, Yuanyuan; Guo, Lin; Wang, Wenhui; Dou, Meijie; Deng, Youjin; Ming, Ray; Zhang, Jisen

Permalink

https://hdl.handle.net/2142/105494

Description

Title
Mitochondrial genome in Hypsizygus marmoreus and its evolution in Dikarya
Author(s)
  • Wang, Gang
  • Lin, Jingxian
  • Shi, Yang
  • Chang, Xiaoguang
  • Wang, Yuanyuan
  • Guo, Lin
  • Wang, Wenhui
  • Dou, Meijie
  • Deng, Youjin
  • Ming, Ray
  • Zhang, Jisen
Issue Date
2019-10-22
Keyword(s)
  • Hypsizygus marmoreus
  • Basidiomycota
  • Mitochondrial genome
  • SNP
  • Dikarya
Abstract

Background

Hypsizygus marmoreus, a high value commercialized edible mushroom is widely cultivated in East Asia, and has become one of the most popular edible mushrooms because of its rich nutritional and medicinal value. Mitochondria are vital organelles, and play various essential roles in eukaryotic cells.


Results

In this study, we provide the Hypsizygus marmoreus mitochondrial (mt) genome assembly: the circular sequence is 102,752 bp in size and contains 15 putative protein-coding genes, 2 ribosomal RNAs subunits and 28 tRNAs. We compared the mt genomes of the 27 fungal species in the Pezizomycotina and Basidiomycotina subphyla, with the results revealing that H. marmoreus is a sister to Tricholoma matsutake and the phylogenetic distribution of this fungus based on the mt genome. Phylogenetic analysis shows that Ascomycetes mitochondria started to diverge earlier than that of Basidiomycetes and supported the robustness of the hyper metric tree. The fungal sequences are highly polymorphic and gene order varies significantly in the dikarya data set, suggesting a correlation between the gene order and divergence time in the fungi mt genome. To detect the mt genome variations in H. marmoreus, we analyzed the mtDNA sequences of 48 strains. The phylogeny and variation sited type statistics of H. marmoreus provide clear-cut evidence for the existence of four well-defined cultivations isolated lineages, suggesting female ancestor origin of H. marmoreus. Furthermore, variations on two loci were further identified to be molecular markers for distinguishing the subgroup containing 32 strains of other strains. Fifteen conserved protein-coding genes of mtDNAs were analyzed, with fourteen revealed to be under purifying selection in the examined fungal species, suggesting the rapid evolution was caused by positive selection of this gene.


Conclusions

Our studies have provided new reference mt genomes and comparisons between species and intraspecies with other strains, and provided future perspectives for assessing diversity and origin of H. marmoreus.

Publisher
BMC. Springer Nature.
Series/Report Name or Number
BMC Genomics; vol. 20, October 2019
Type of Resource
text
Language
en
Permalink
http://hdl.handle.net/2142/105494
DOI
https://doi.org/10.1186/s12864-019-6133-z
Copyright and License Information
Copyright 2019 Gang Wang, Jingxian Lin, Yang Shi, Xiaoguang Chang, Yuanyuan Wang, Lin Guo, Wenhui Wang, Meijie Dou, Youjin Deng, Ray Ming, and Jisen Zhang

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