University of Illinois Urbana-Champaign

Mechanistic interrogations of ribosomal and non-ribosomal natural product enzymes

Cogan, Dillon P.

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https://hdl.handle.net/2142/105160

Description

Title
Mechanistic interrogations of ribosomal and non-ribosomal natural product enzymes
Author(s)
Cogan, Dillon P.
Issue Date
2019-04-08
Director of Research (if dissertation) or Advisor (if thesis)
Nair, Satish K.
Doctoral Committee Chair(s)
Nair, Satish K.
Committee Member(s)
  • van der Donk, Wilfred A.
  • Gerlt, John A.
  • Rienstra, Chad M.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
  • biosynthesis
  • natural product
  • enzyme
  • RiPP
  • ribosomal
  • peptide
  • polyketide
  • non-ribosomal
  • DNA-binding
  • thiopeptide
  • lanthipeptide
Abstract
Described herein is my thesis work towards structural and biochemical characterization of bacterial proteins involved in metabolite regulation and natural product biosynthesis. In the first case, I will describe the structural basis for transcriptional regulation of a small molecule metabolite, p-coumarate, and the remaining cases involve enzymes responsible for biosyntheses of complex natural products. Among the natural product cases are those falling under the class of ribosomally synthesized and post-translationally modified peptides (RiPPs), with a particular emphasis on the thiopeptide subclass of RiPPs. Additionally, characterization of an off-loading enzyme involved in hybrid polyketide/non-ribosomal peptide (PK-NRP) natural product biosynthesis is described.
Graduation Semester
2019-05
Type of Resource
text
Permalink
http://hdl.handle.net/2142/105160
Copyright and License Information
Copyright 2019 Dillon Cogan

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