University of Illinois Urbana-Champaign

Quantitative characterization of cellular dynamics in wound healing using multimodal microscopy

Li, Joanne

Content Files
LI-DISSERTATION-2019.pdf

Permalink

https://hdl.handle.net/2142/104958

Description

Title
Quantitative characterization of cellular dynamics in wound healing using multimodal microscopy
Author(s)
Li, Joanne
Issue Date
2019-01-23
Director of Research (if dissertation) or Advisor (if thesis)
Boppart, Stephen A.
Doctoral Committee Chair(s)
Boppart, Stephen A.
Committee Member(s)
  • Boppart, Marni M.
  • Dobrucki, Wawrzyniec L.
  • Smith, Andrew M.
Department of Study
Bioengineering
Discipline
Bioengineering
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Date of Ingest
2019-08-23T20:28:04Z
Keyword(s)
Optical imaging, Biomedical imaging, Microscopy, Wound healing
Abstract
In this thesis, cellular dynamics in skin under various pathological conditions were characterized utilizing multimodal multiphoton and optical coherence microscopy (MPM OCM). Through these studies, additional insights were gained regarding the complex relationships among different skin constituents, and how abnormal pathological states can easily disrupt the homeostasis in the skin microenvironment. At the molecular level, the robustness and cellular resolution of multimodal MPM OCM enabled longitudinal tracking of the effect of recombinant interleukin on the chemical environment in wounded skin. At the cellular level, the efficacy of stem cell treatment on accelerating wound closure on diabetic skin was characterized. Most importantly, the effectiveness of nonlinear microscopy on visualizing relationship between administered cells and local host environment was clearly demonstrated. In addition, the potential of multimodal MPM OCM as a screening tool for pharmaceutical treatment was demonstrated through analyzing the mechanisms of a novel topical ointment on stimulating angiogenesis in non-healing diabetic wounds. Though nonlinear microscopy possesses great potentials as a clinical diagnostic imaging system, significant limitations were revealed in this thesis that the skin miroenvironment cannot be further understood without technology capable of characterizing cellular dynamics in ways similar to the native environment. To address this challenge, a video-rate multimodal microscopy system was developed, together with a multimodal 3D image analysis platform. A pilot study was performed to demonstrate the combination of high-speed imaging and 3D analysis tool can potentially reinvent the way pathological environment of diseases were characterized. By improving current imaging and analytical techniques for studying diseases in their natural states, optical imaging technology may have broader impact on biomedical research and natural sciences.
Graduation Semester
2019-05
Type of Resource
text
Permalink
http://hdl.handle.net/2142/104958
Copyright and License Information
Copyright 2019 Joanne Li

Owning Collections

Graduate Dissertations and Theses at Illinois PRIMARY
Graduate Theses and Dissertations at Illinois
Dissertations and Theses - Bioengineering