Mapping the Nucleus

Su, Christina

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https://hdl.handle.net/2142/103803 Copy

Description

Title

Mapping the Nucleus

Author(s)

Su, Christina

Contributor(s)

Tasan, Ipek

Issue Date

2019

Keyword(s)

• Molecular and Cellular Biology
• Map
• Nucleome
• DNA

Abstract

DNA is not just randomly “stuffed” inside the nucleus of cells. Instead, it is highly organized! Similar to how we map our earth, we want to construct a map of the nucleus. One mapping method is to find repeated segments within the genome and bind compatible fluorescent markers. This would be like finding all of the twins, triplets, and other multiplets on earth and having them wear special fluorescent hats (A). However, just like for humans, finding these repeats naturally within the cell is pretty rare. Instead, we use a technique that synthetically produces these multiplets and markers and we are not limited to just twins and triplets, but can create segments up to 96 repeats long- 96tuplet! Once introduced, we are able to visualize the location of specific segments of DNA within the cell (B). Shown is what the actual labeling looks like within the nucleus of a cell! Ultimately, our goal as humans is to understand the locations of countries and their relationships with each other over time. Similarly, this nuclear map will help us understand the interactions between different areas and how this may affect the cell’s processes in disease, under normal conditions, and more.

Type of Resource

• text
• image

Permalink

http://hdl.handle.net/2142/103803

Copyright and License Information

Copyright 2019 Christina Su

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