University of Illinois Urbana-Champaign

Characteristics of sperm binding to the mouse oviduct

Doungkamchan, Kankanit

Content Files
DOUNGKAMCHAN-THESIS-2018.pdf
Supplemental movie 10 (pig sperm).avi
Supplemental movie 11 (mouse sperm).avi
Supplemental movie 1 (isthmus).avi
Supplemental movie 2 (ampulla).avi
Supplemental movie 3 (trachea).avi
Supplemental movie 4 (non-capacitated sperm).avi
Supplemental movie 5 (capacitated sperm).avi
Supplemental movie 6 (non-viscous medium).avi
Supplemental movie 7 (viscous medium).avi
Supplemental movie 8 (87 weeks old)).avi
Supplemental movie 9 (13 weeks old).avi

Permalink

https://hdl.handle.net/2142/102833

Description

Title
Characteristics of sperm binding to the mouse oviduct
Author(s)
Doungkamchan, Kankanit
Issue Date
2018-12-06
Director of Research (if dissertation) or Advisor (if thesis)
Miller, David J.
Committee Member(s)
  • Bahr, Janice M.
  • Knox, Robert V.
Department of Study
Animal Sciences
Discipline
Animal Sciences
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
M.S.
Degree Level
Thesis
Date of Ingest
2019-02-07T20:44:21Z
Keyword(s)
Sperm, Oviduct, Reservoir
Abstract
A wide variety of animals store sperm between mating and ovulation. The sperm reservoir prolongs sperm lifespan and keeps sperm in a quiescent stage due to delayed capacitation and suppressed motility. The objective of this study was to determine different characteristics of sperm binding to the oviduct. The study was conducted using the translucent mouse oviduct ex vivo. The specificity of sperm binding was determined using the isthmus, ampulla, and trachea (n = 4). The results showed no difference in binding between isthmus and trachea (P > 0.05) but binding to the ampulla was less than the isthmus (P < 0.05). Capacitation status also affected sperm binding (n = 3). The number of sperm from non-capacitated samples bound to the isthmus was significantly higher than the number of bound capacitated sperm (P < 0.05). Despite increased stiffness of oviduct cilia as female age, the number of sperm bound to the isthmus of older females was higher than younger females (P < 0.05). The number of bound sperm was different throughout estrous stages (P < 0.05) with diestrus being the highest (n = 3). Species-specific binding was also examined by allowing porcine and mouse sperm to bind the mouse isthmus (n =3). A significantly higher number of mouse sperm bound to the mouse isthmus than porcine sperm (P < 0.05). In conclusion, sperm binding to the oviduct ex vivo was not completely specific to oviduct epithelial cells or age-dependent but was species-restricted, capacitation-dependent and dependent on the stage of estrous.
Graduation Semester
2018-12
Type of Resource
text
Permalink
http://hdl.handle.net/2142/102833
Copyright and License Information
Copyright 2018 Kankanit Doungkamchan

Owning Collections

Graduate Dissertations and Theses at Illinois PRIMARY
Graduate Theses and Dissertations at Illinois
Dissertations and Theses - Animal Sciences