In situ chemical characterization of the motile to sessile transition of psedomonas aeruginosa communities

Cao, Tianyuan

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https://hdl.handle.net/2142/100832 Copy

Description

Title

In situ chemical characterization of the motile to sessile transition of psedomonas aeruginosa communities

Author(s)

Cao, Tianyuan

Contributor(s)

• Bohn, Paul W.
• Shrout, Joshua D.
• Baig, Nameera F.
• Kramer, Kristen M.
• Morales-Soto, Nydia

Issue Date

2018-06-21

Keyword(s)

Spectroscopy as an analytical tool

Abstract

Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen which infects more than 50,000 people each year in the United States alone. Its abilities to move, colonize surfaces, and develop biofilms give rise to the high resistance to antimicrobial treatment. One type of motility employed by P. aeruginosa is swarming motility, where bacterial cells undergo physical and metabolic alterations. Swarming has been studied by many researchers but the knowledge on its chemical composition that relates to the transition between the motile and sessile biofilm stages are still lack. Here we apply confocal Raman microscopy (CRM) to examine P. aeruginosa wild-type PA14 (a virulent strain isolated from a burn wound) under swarming and biofilm conditions. The comparison between the swarming and biofilm samples indicates different molecules linked to the motile to sessile transition, revealing their community-specific chemical features. While the Pseudomonas quinolone signal (PQS) is found in swarm colonies and biofilms, the N-oxide quinolines (4-hydroxy-2-heptylquinoline-N-oxide,2-nonyl-4- hydroxyquinoline, etc.) are present in higher abundance and are synthesized and secreted much earlier in swarm colonies. Moreover, a closer investigation spanning from the center to the edge of a swarm colony shows high abundance of PQS at the center while N-oxides dominate the edge of the colony. The results provide insights into the chemical profile change occurring during the motile to sessile transition in P. aeruginosa, and demonstrate the broad application of CRM in biomolecular imaging.

Publisher

International Symposium on Molecular Spectroscopy

Type of Resource

text

Language

eng

Permalink

http://hdl.handle.net/2142/100832

DOI

10.15278/isms.2018.RH08

Copyright and License Information

Copyright 2018 Tianyuan Cao

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