Single-shot sub-microsecond spectroscopy of the bacteriorhodopsin photocycle with quantum cascade laser frequency combs

Mangold, Markus

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https://hdl.handle.net/2142/100785 Copy

Description

Title

Single-shot sub-microsecond spectroscopy of the bacteriorhodopsin photocycle with quantum cascade laser frequency combs

Author(s)

Mangold, Markus

Contributor(s)

• Faist, Jerome
• Allmendinger, Pitt
• Hugi, Andreas
• Klocke, Jessica
• Kottke, Tilman

Issue Date

2018-06-19

Keyword(s)

Mini-symposium: Frequency-Comb Spectroscopy

Abstract

Time-resolved vibrational spectroscopy is an important tool for understanding biological processes and chemical reaction pathways [1]. Today, all available methods to our knowledge require many repetitions of an experiment to acquire a microsecond time-resolved mid-infrared spectrum. We present the IRspectrometer, a quantum cascade laser dual frequency comb spectrometer [2-3]. It allows for parallel acquisition of hundreds of mid-infrared wavelengths with microsecond time resolution. The formation of the light-activated L, M and N-states in bacteriorhodopsin – which only have µs to ms lifetimes – has been recorded by analyzing the infrared response of bacteriorhodopsin to 10 ns visible light pulses with sub-microsecond time-resolution. The different wavelengths were all measured in parallel thanks to the dual-comb approach. The spectra as well as the kinetics show good agreement with those from step-scan FT-IR measurements. As a benchmark, the spectral signature of several intermediate states of the bacteriorhodopsin photocycle has been recorded in a single shot measurement. This approach greatly reduces the complexity of time-resolved spectroscopy measurements in the mid-infrared which currently require many repetitions. References [1] Ritter, E. et al. “Time-Resolved Infrared Spectroscopic Techniques as Applied to Channelrhodopsin.” Front. Mol. Biosci. 2:38. [2] Hugi, A. et al. “Mid-Infrared Frequency Comb Based on a Quantum Cascade Laser.” Nature 492: 229–33 [3] Villares, G. et al. “Dual-comb spectroscopy based on quantum-cascade-laser frequency combs.” Nat. Commun. 5:5192

Publisher

International Symposium on Molecular Spectroscopy

Type of Resource

text

Language

eng

Permalink

http://hdl.handle.net/2142/100785

DOI

10.15278/isms.2018.TG03

Copyright and License Information

Copyright 2018 Markus Mangold

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