University of Illinois Urbana-Champaign

Development of spectroscopic smartphone biosensors for point-of-care applications

Long, Kenneth D.

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https://hdl.handle.net/2142/101252

Description

Title
Development of spectroscopic smartphone biosensors for point-of-care applications
Author(s)
Long, Kenneth D.
Issue Date
2018-01-31
Director of Research (if dissertation) or Advisor (if thesis)
Cunningham, Brian T.
Doctoral Committee Chair(s)
Cunningham, Brian T.
Committee Member(s)
  • Bashir, Rashid
  • Boppart, Stephen A.
  • Achenbach, Chad J.
Department of Study
Bioengineering
Discipline
Bioengineering
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
  • Smartphone biosensing
  • point-of-care testing
  • photonic crystal
  • photonic crystal enhanced microscopy
  • digital detection
  • smartphone spectroscopy
  • HIV viral load
  • smartphone diagnostics
  • POCT
  • PCEM
  • single nanoparticle detection
  • TRI-Analyzer
  • Spectral TRI-Analyzer
Abstract
This dissertation explores the combination of two emergent areas within contemporary biosensing, smartphone based spectroscopy and photonic crystal enhanced microscopy, and how these technologies can be combined to produce a fundamentally novel point-of-care testing paradigm: a portable device platform capable of non-amplifying, digital-detection for high-sensitivity diagnostics. In this work, I describe the development of this system, moving from usage-specific benchtop and smartphone based devices demonstrating proof-of-concept capabilities, to a multimodal smartphone platform compatible with thousands of existing spectroscopic assays. The resulting smartphone biosensor can perform various clinically-relevant tests with physiologically-relevant sensitivities. Next, photonic crystal enhanced microscopy is described for uses in the micrometer and nanometer scales for use both to study cellular and subcellular behavior and also to perform single-particle attachment quantification. Finally, this work explores how the single-particle attachment quantification capability can be leveraged to measure HIV viral load using a novel biosensor, designed specifically developed for a smartphone based platform for point of care applications.
Graduation Semester
2018-05
Type of Resource
text
Permalink
http://hdl.handle.net/2142/101252
Copyright and License Information
Copyright 2018 Kenneth D. Long

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Graduate Dissertations and Theses at Illinois PRIMARY
Graduate Theses and Dissertations at Illinois
Dissertations and Theses - Bioengineering