Stabilization of liposomal functional anchors by cross-linkable lipids

Smith, Cartney

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https://hdl.handle.net/2142/100421 Copy

Description

Title

Stabilization of liposomal functional anchors by cross-linkable lipids

Author(s)

Smith, Cartney

Issue Date

2013-07-15

Director of Research (if dissertation) or Advisor (if thesis)

Kong, Hyunjoon

Committee Member(s)

Kong, Hyunjoon

Department of Study

Chemical & Biomolecular Engr

Discipline

Chemical Engineering

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

M.S.

Degree Level

Thesis

Keyword(s)

• Cross-Linkable Lipids
• Magnetic Resonance Imaging
• Chitosan
• Liposome

Abstract

Micro- and nanoparticles are designed to deliver drugs and contrast agents to their target site in a controlled manner. One of the greatest obstacles in the performance of such delivery vehicles is their stability in the presence of serum. Here we investigate a method to stabilize a class of liposomes in which lipid vesicles are modified post- fabrication through surface adsorption and anchoring. We hypothesized that the sequential adsorption of functional units followed by covalent cross-linking of the liposome would provide enhanced stability in the presence of human serum. To investigate this hypothesis, liposomes composed of diyne-containing lipids were assembled and functionalized via chitosan conjugated with a hydrophobic anchor and the magnetic resonance (MR) contrast agent, gadolinium, as a model functionality. This strategy served to stabilize the thermodynamically favorable association between liposome and modified functional chitosan. Furthermore, the chitosan-coated, cross-linked liposomes proved more effective as delivery vehicles of MR contrast agents than uncross-linked liposomes due to the reduced liposome degradation and chitosan desorption. Overall, this study demonstrates a useful method to stabilize a broad class of particles used for systemic delivery of various molecular cargos.

Graduation Semester

2013-08

Type of Resource

text

Permalink

• http://hdl.handle.net/123456789/2295
• http://hdl.handle.net/2142/100421

Copyright and License Information

Copyright 2013 Cartney E. Smith

Owning Collections