Discovery of Glycocin from Pathway Refactoring

Ho, Sherri; Ren, Hengqian

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https://hdl.handle.net/2142/99957 Copy

Description

Title

Discovery of Glycocin from Pathway Refactoring

Author(s)

• Ho, Sherri
• Ren, Hengqian

Contributor(s)

Zhao, Huimin

Issue Date

2018-04

Keyword(s)

• Molecular and Cellular Biology
• Synthetic Biology
• Science
• Natural Product
• Glycocin
• Protein Purification

Abstract

Natural products have been playing an indispensable role in pharmaceuticals and human therapeutics for decades. However, because of the continuous development of drug resistance, high throughput discovery of natural products is always an urgent but seemingly impossible challenge. Synthetic biology, with its power to engineer biosynthetic pathways, has proven to be a promising solution to this issue. One type of natural product that will be investigated are ribosomally synthesized and post-translationally modified peptide (RiPP) natural products. Glycocins, a class of RiPPs, are a form of bacterial toxins that can inhibit the growth of many bacterial strains. In this study, we used heterologous expression to engineer the pathway of glycocin from Geobacillus followed by fermentation, fractionation, and purification of the glycocin. In the end, we discovered a glycocin from the thermophilic bacteria Geobacillus which has never been done before.

Type of Resource

image

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http://hdl.handle.net/2142/99957

Sponsor(s)/Grant Number(s)

MCB SROP

Copyright and License Information

• Copyright 2018 Sherri Ho
• Copyright 2018 Hengqian Ren

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