Multiplexed detection of infectious pathogens using on-chip loop-mediated isothermal amplification and a smartphone
Sun, Fu
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https://hdl.handle.net/2142/99239
Description
Title
Multiplexed detection of infectious pathogens using on-chip loop-mediated isothermal amplification and a smartphone
Author(s)
Sun, Fu
Issue Date
2017-12-06
Director of Research (if dissertation) or Advisor (if thesis)
Cunningham, Brian T.
Department of Study
Electrical & Computer Eng
Discipline
Electrical & Computer Engr
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
M.S.
Degree Level
Thesis
Keyword(s)
Detection
Pathogens
Point-of-care
Abstract
New tools are needed to enable rapid detection, identification, and reporting of infectious pathogens in a wide variety of point-of-care applications that impact human and animal health. In this work, we report the design, construction, and characterization of a platform for multiplexed analysis of disease-specific DNA sequences that utilizes a smartphone camera as the sensor in conjunction with a handheld instrument that interfaces the phone with a silicon-based microfluidic chip. Utilizing specific nucleic acid sequences for four equine respiratory pathogens as representative examples, we demonstrated the ability of the system to use a single droplet of test sample to perform selective qualitative determination of target sequences with integrated experimental controls in an hour. The system achieves detection limits comparable to those obtained by laboratory-based methods and instruments.
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