Advanced applications of vibrational circular dichroism: From small chiral molecules to fibrils

Dukor, Rina K.

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https://hdl.handle.net/2142/97129 Copy

Description

Title

Advanced applications of vibrational circular dichroism: From small chiral molecules to fibrils

Author(s)

Dukor, Rina K.

Issue Date

2017-06-22

Keyword(s)

Chirality-sensitive spectroscopy

Abstract

Vibrational Circular Dichroism (VCD), first discovered in the early 1970s, and commercialized in the late 1990's, is finally coming of age! No longer a curiosity of the few selected academic groups, it is now used by all major pharmaceutical companies, regulatory agencies, government labs and academic institutions. The main application for the technology has been determination of absolute configuration of small pharmaceutical molecules. In more recent years, this has extended to more complicated molecules such as natural products with many chiral centers and conformational flexibility. Other applications include determination of enantiomeric purity, chiral polymers, and characterization of other biological molecules such as proteins, carohydrates and nucleic acids. _x000d_ _x000d_ One of the most fascinating discoveries in the VCD field has been been unusual enhancement in intensity for proteins that form fibrils. We have demonstrated sensitivity of VCD to in situ solution-phase probe of the process of fibrillogenesis and subsequent development that currently can only be studied in detail with dried samples by such techniques as scanning electron microscopy or atomic force microscopy. We have further shown that several different proteins, that in their native state have different secondary structures, have a very similar unique signature of mature fibrils. _x000d_ _x000d_ In this presentation, we will discuss fundamentals of VCD, demonstrate a few examples of different applications and showcase the sensitivity to structure of fibrils, including new results on micro-sampling.

Publisher

International Symposium on Molecular Spectroscopy

Type of Resource

text

Language

eng

Permalink

http://hdl.handle.net/2142/97129

DOI

https://doi.org/10.15278/isms.2017.RG07

Copyright and License Information

Copyright 2017 Rina K. Dukor

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