S-nitrosothiols observed using cavity ring-down spectroscopy

Rad, Mary Lynn

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https://hdl.handle.net/2142/96993 Copy

Description

Title

S-nitrosothiols observed using cavity ring-down spectroscopy

Author(s)

Rad, Mary Lynn

Contributor(s)

• Lehmann, Kevin
• Gaston, Benjamin M.

Issue Date

2017-06-21

Keyword(s)

Spectroscopy as an analytical tool

Abstract

The biological importance of nitric oxide has been known for nearly forty years due to its role in cardiovascular and nervous signaling. The main carrier molecules, s-nitrosothiols (RSNOs), are of additional interest due to their role in signaling reactions. Additionally, these compounds are related to several diseases including muscular dystrophy, stroke, myocardial infarction, Alzheimer's disease, Parkinson's disease, cystic fibrosis, asthma, and pulmonary arterial hypertension. One of the main barriers to elucidating the role of these RSNOs is the low (nanomolar) concentration present in samples of low volume (typically $sim$100 $mu$L). To this end we have set up a cavity ring-down spectrometer tuned to observe $^{14}$NO and $^{15}$NO released from cell growth samples. To decrease the limit of detection we have implemented a laser locking scheme employing Zeeman modulation of NO in a reference cell and have tuned the polarization of the laser using a half wave plate to optimize the polarization for the inherent birefringence of the CRDS mirrors. Progress toward measuring RSNO concentration in biological samples will be presented.

Publisher

International Symposium on Molecular Spectroscopy

Type of Resource

text

Language

eng

Permalink

http://hdl.handle.net/2142/96993

DOI

https://doi.org/10.15278/isms.2017.WE03

Copyright and License Information

Copyright 2017 Mary Lynn Rad

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