University of Illinois Urbana-Champaign

Noise in noise out - single molecule studies of molecular motors

Tjioe, Marco

Content Files
TJIOE-DISSERTATION-2017.pdf

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https://hdl.handle.net/2142/98156

Description

Title
Noise in noise out - single molecule studies of molecular motors
Author(s)
Tjioe, Marco
Issue Date
2017-05-26
Director of Research (if dissertation) or Advisor (if thesis)
Selvin, Paul R.
Doctoral Committee Chair(s)
Selvin, Paul R.
Committee Member(s)
  • Chemla, Yann R.
  • DeMarco, Brian L.
  • Zhang, Kai
Department of Study
School of Molecular & Cell Bio
Discipline
Biophysics & Computnl Biology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Date of Ingest
2017-09-29T17:45:38Z
Keyword(s)
  • Single molecule
  • Kinesin
  • Dynein
  • Noise
  • Frames per step (FPS)
  • Magnetic purification
  • Signal-to-noise ratio
  • Molecular motor
Abstract
Many cellular functions rely on the movements of molecular motors. Single molecule studies of these motors allow investigation into their step-sizes and stall forces. Such studies inevitably involve noise, which is inherent in the biological and imaging systems probing these motors. Having to work with noise in the system, we develop a parameter, called the FIONA Index, which serves as a guide for data quality based on noise per step-size (NPS) and frames per step (FPS). Attempting to bring the noise out of the system, we develop Magnetic Cytoskeleton Affinity (MiCA) purification to remove excess dyes that prevent clear observation of individual motors. We show that with appropriate treatment of noise, complex systems can be assembled to study how multiple motors work together.
Graduation Semester
2017-08
Type of Resource
text
Permalink
http://hdl.handle.net/2142/98156
Copyright and License Information
Copyright 2017 Marco Tjioe

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