Methods for Measuring Dry Mass Change in Time-lapse Gradient Light Interference Microscopy
Author(s)
Carrol, Brittani L.
Kandel, Mikhail Eugene
Kouzehgarani, Ghazal Naseri
Gillette, Martha U.
Popescu, Gabriel
Issue Date
2017
Keyword(s)
Gradient light interference microscopy (GLIM)
quantitative
Abstract
Gradient Light Interference Microscopy (GLIM) is a recently developed label-free technique used for imaging optically thick samples, such as acute brain slices. GLIM is similar to spatial light interference microscopy (SLIM) but differs from it because it measures the gradient of the phase rather than the phase itself [2]. Once the sample is imaged, it needs to be reconstructed, meaning phase integration of the gradient image must be performed to recover the object’s scattering potential. In this project, we validated the performance of GLIM by comparing two integration techniques with samples of pillars as well as with acute brain slices. We demonstrated the capability of our approach to measuring the dry mass change of cells in the brain slices over periods of the order of hours and developed a new imaging method, GLIM, for imaging 3D tissue cultures and models, which enables quantitative observation with DIC images, and has the potential to track subtle changes in diseased tissue.
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