Liver Organoid Assembly for Toxicity Assessment of Silver Nanoparticles
Bidon, Miya K.; Sullivan, Kathryn M.; Park, Jooyen; Kong, Hyunjoon
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https://hdl.handle.net/2142/98074
Description
Title
Liver Organoid Assembly for Toxicity Assessment of Silver Nanoparticles
Author(s)
Bidon, Miya K.
Sullivan, Kathryn M.
Park, Jooyen
Kong, Hyunjoon
Issue Date
2017
Keyword(s)
Silver nanoparticles
3D culture
HepG2
extracellular matrices
mescenchymal stem cells
Abstract
Due to their interaction with water, nanoparticles experience a compounding cycle of being absorbed in multiple organisms, most notably commercial fish and humans. The purpose of this research is to assemble clusters of hepatic cells to enable the assessment of the toxicity levels of silver nanoparticles (AgNPs). For this analysis, we cultured liver cancer cells (HepG2) into decellularized MSC matrices onto non-adherent surfaces to maintain cellular phenotype, increase cell proliferation, and promote high differentiation potential. Based on this research, spheroid MSC-DCM has shown to provide more physiologically relevant conditions to enhance the growth and detoxification activity of liver cancer cells. From this, we were able to quantitatively and qualitatively analyze the increased toxicity levels of AgNPs between size 40 and 130 nm.
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