Surface chemistry of carbon nanoparticles functionally select their uptake in various stages of cancer cells

Srivastava, Indrajit

Permalink

https://hdl.handle.net/2142/97709 Copy

Description

Title

Surface chemistry of carbon nanoparticles functionally select their uptake in various stages of cancer cells

Author(s)

Srivastava, Indrajit

Issue Date

2017-04-26

Director of Research (if dissertation) or Advisor (if thesis)

Pan, Dipanjan

Department of Study

Bioengineering

Discipline

Bioengineering

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

M.S.

Degree Level

Thesis

Keyword(s)

• Personalized medicine
• Endocytosis
• Surface chemistry
• Carbon nanoparticles

Abstract

Relationship of the surface physicochemical characteristics of nanoparticles with their interactions with biological entities may provide critical information for nanomedicinal applications. In this work, we have presented the systematic synthesis of sub 50nm carbon nanoparticles (CNP) presenting neutral, anionic, and cationic surface headgroups. A subset of CNPs with ~ 10, 20, and 40nm hydrodynamic sizes are synthesized with neutral surface headgroups. The cellular internalization of these CNPs was systematically quantified for the first time in various stages of breast cancer cells (early, late and metastatic), providing a parametric assessment of charge and size effects. Distinct activities are noticed with these systems as they interact with various stages of the cancer cells. Our results indicated that a metastatic breast cancer could be targeted with a nanosystem presenting anionic phosphate groups. On the contrary, for patients with late stage cancer, drugs could be delivered with sulfonate functionalized carbon nanoparticles with higher probability of intracellular transport. This study will facilitate a better understanding of nanoparticle-biologic interaction and the integration of this knowledge with pathophysiology would help to engineer nanomedicine with superior likelihoods to cross the endocytic “barrier” for delivering drug inside the cancerous cells.

Graduation Semester

2017-05

Type of Resource

text

Permalink

http://hdl.handle.net/2142/97709

Copyright and License Information

Copyright 2017 Indrajit Srivastava

Owning Collections